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hcd46  (R&D Systems)


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    Structured Review

    R&D Systems hcd46
    Hcd46, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd46+antibodies/Human+CD46+Antibody/pm41989164-105-20-22
    Average 92 stars, based on 12 article reviews
    hcd46 - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Staining:

    Article Title: Modulators and methods of use
    Article Snippet: Additionally, both cell lines were stained with anti-CD46 antibodies conjugated to the fluorophore phycoerythrin (R&D Systems) and analyzed by flow cytometry to demonstrate downregulation of CD46 expression in lentivirally transduced (and sorted) cell line. .. Additionally, both cell lines were stained with anti-CD46 antibodies conjugated to the fluorophore phycoerythrin (R&D Systems) and analyzed by flow cytometry to demonstrate downregulation of CD46 expression in lentivirally transduced (and sorted) cell line. ..

    Article Title: Structural basis for HCMV Pentamer receptor recognition and antibody neutralization
    Article Snippet: .. For cell surface staining, adherent cells (ARPE-19, HUVEC, and HAP-1) were gently detached with a cell scraper, washed twice with PBS + 2% FBS, and incubated in PBS + 0.5% bovine serum albumin (BSA) + 2 mM EDTA with specific anti-NRP2 and anti-CD46 antibodies (AF2215 and AF2005, R&D Systems), anti-THBD antibody (clone 141C01, Abcam), or, as isotype control, normal sheep immunoglobulin G (IgG) affinity pure (R&D Systems) at 2 μg/ml for 30 min on ice. .. After two washes, cells were incubated with anti-mouse IgG (H+L) Alexa Fluor 594–conjugated (Thermo Fisher Scientific) secondary antibodies at 2 mg/ml for 30 min on ice, washed twice, and acquired with a FACS (fluorescence-activated cell sorting) Fortessa (BD Biosciences) flow cytometer.

    Article Title: Modulators and methods of use
    Article Snippet: Additionally, both cell lines were stained with anti-CD46 antibodies conjugated to the fluorophore phycoerythrin (R&D Systems) and analyzed by flow cytometry to demonstrate downregulation of CD46 expression in lentivirally transduced (and sorted) cell line. .. Additionally, both cell lines were stained with anti-CD46 antibodies conjugated to the fluorophore phycoerythrin (R&D Systems) and analyzed by flow cytometry to demonstrate downregulation of CD46 expression in lentivirally transduced (and sorted) cell line. ..

    Article Title: An Unbiased Screen for Human Cytomegalovirus Identifies Neuropilin-2 as a Central Viral Receptor.
    Article Snippet: .. For cell-surface staining, adherent cells (ARPE-19, HAP-1, HUVEC andMRC-9) were gently detachedwith a cell scraper andwashed twice with PBS+2% FBS, incubated in PBS + 0.5% BSA + 2 mM EDTA with specific anti-Neuropilin-2 and anti-CD46 antibodies (AF2215 and AF2005, R&D systems), anti-Thrombomodulin antibody (clone 141C01, Abcam), anti-TGFbRIII antibody (T1940, Sigma) or, as isotype control, normal sheep IgG affinity pure (R&D systems), goat a-CMV pp71 (vC-20; Santa Cruz Biotechnology) and mouse IgG1 a-CMV pp72 (clone 6E1; Santa Cruz Biotechnology) at 2 mg/ml for 30 min on ice. .. After two washes, cells were incubated with rabbit anti-goat IgG (H+L) Alexa Fluor 594 conjugated or donkey anti-sheep IgG (H+L) Alexa Fluor 488 conjugated or goat F(ab)2 anti-mouse IgG (H+L) Alexa Fluor 594 conjugated (Thermo Fisher Scientific) secondary antibodies at 2 mg/ml for 30 min on ice, washed twice and acquired with a FACS Fortessa (BD Biosciences) flow cytometer.

    Flow Cytometry:

    Article Title: Modulators and methods of use
    Article Snippet: Additionally, both cell lines were stained with anti-CD46 antibodies conjugated to the fluorophore phycoerythrin (R&D Systems) and analyzed by flow cytometry to demonstrate downregulation of CD46 expression in lentivirally transduced (and sorted) cell line. .. Additionally, both cell lines were stained with anti-CD46 antibodies conjugated to the fluorophore phycoerythrin (R&D Systems) and analyzed by flow cytometry to demonstrate downregulation of CD46 expression in lentivirally transduced (and sorted) cell line. ..

    Article Title: Modulators and methods of use
    Article Snippet: Additionally, both cell lines were stained with anti-CD46 antibodies conjugated to the fluorophore phycoerythrin (R&D Systems) and analyzed by flow cytometry to demonstrate downregulation of CD46 expression in lentivirally transduced (and sorted) cell line. .. Additionally, both cell lines were stained with anti-CD46 antibodies conjugated to the fluorophore phycoerythrin (R&D Systems) and analyzed by flow cytometry to demonstrate downregulation of CD46 expression in lentivirally transduced (and sorted) cell line. ..

    Expressing:

    Article Title: Modulators and methods of use
    Article Snippet: Additionally, both cell lines were stained with anti-CD46 antibodies conjugated to the fluorophore phycoerythrin (R&D Systems) and analyzed by flow cytometry to demonstrate downregulation of CD46 expression in lentivirally transduced (and sorted) cell line. .. Additionally, both cell lines were stained with anti-CD46 antibodies conjugated to the fluorophore phycoerythrin (R&D Systems) and analyzed by flow cytometry to demonstrate downregulation of CD46 expression in lentivirally transduced (and sorted) cell line. ..

    Article Title: Modulators and methods of use
    Article Snippet: Additionally, both cell lines were stained with anti-CD46 antibodies conjugated to the fluorophore phycoerythrin (R&D Systems) and analyzed by flow cytometry to demonstrate downregulation of CD46 expression in lentivirally transduced (and sorted) cell line. .. Additionally, both cell lines were stained with anti-CD46 antibodies conjugated to the fluorophore phycoerythrin (R&D Systems) and analyzed by flow cytometry to demonstrate downregulation of CD46 expression in lentivirally transduced (and sorted) cell line. ..

    Incubation:

    Article Title: Structural basis for HCMV Pentamer receptor recognition and antibody neutralization
    Article Snippet: .. For cell surface staining, adherent cells (ARPE-19, HUVEC, and HAP-1) were gently detached with a cell scraper, washed twice with PBS + 2% FBS, and incubated in PBS + 0.5% bovine serum albumin (BSA) + 2 mM EDTA with specific anti-NRP2 and anti-CD46 antibodies (AF2215 and AF2005, R&D Systems), anti-THBD antibody (clone 141C01, Abcam), or, as isotype control, normal sheep immunoglobulin G (IgG) affinity pure (R&D Systems) at 2 μg/ml for 30 min on ice. .. After two washes, cells were incubated with anti-mouse IgG (H+L) Alexa Fluor 594–conjugated (Thermo Fisher Scientific) secondary antibodies at 2 mg/ml for 30 min on ice, washed twice, and acquired with a FACS (fluorescence-activated cell sorting) Fortessa (BD Biosciences) flow cytometer.

    Article Title: An Unbiased Screen for Human Cytomegalovirus Identifies Neuropilin-2 as a Central Viral Receptor.
    Article Snippet: .. For cell-surface staining, adherent cells (ARPE-19, HAP-1, HUVEC andMRC-9) were gently detachedwith a cell scraper andwashed twice with PBS+2% FBS, incubated in PBS + 0.5% BSA + 2 mM EDTA with specific anti-Neuropilin-2 and anti-CD46 antibodies (AF2215 and AF2005, R&D systems), anti-Thrombomodulin antibody (clone 141C01, Abcam), anti-TGFbRIII antibody (T1940, Sigma) or, as isotype control, normal sheep IgG affinity pure (R&D systems), goat a-CMV pp71 (vC-20; Santa Cruz Biotechnology) and mouse IgG1 a-CMV pp72 (clone 6E1; Santa Cruz Biotechnology) at 2 mg/ml for 30 min on ice. .. After two washes, cells were incubated with rabbit anti-goat IgG (H+L) Alexa Fluor 594 conjugated or donkey anti-sheep IgG (H+L) Alexa Fluor 488 conjugated or goat F(ab)2 anti-mouse IgG (H+L) Alexa Fluor 594 conjugated (Thermo Fisher Scientific) secondary antibodies at 2 mg/ml for 30 min on ice, washed twice and acquired with a FACS Fortessa (BD Biosciences) flow cytometer.

    Control:

    Article Title: Structural basis for HCMV Pentamer receptor recognition and antibody neutralization
    Article Snippet: .. For cell surface staining, adherent cells (ARPE-19, HUVEC, and HAP-1) were gently detached with a cell scraper, washed twice with PBS + 2% FBS, and incubated in PBS + 0.5% bovine serum albumin (BSA) + 2 mM EDTA with specific anti-NRP2 and anti-CD46 antibodies (AF2215 and AF2005, R&D Systems), anti-THBD antibody (clone 141C01, Abcam), or, as isotype control, normal sheep immunoglobulin G (IgG) affinity pure (R&D Systems) at 2 μg/ml for 30 min on ice. .. After two washes, cells were incubated with anti-mouse IgG (H+L) Alexa Fluor 594–conjugated (Thermo Fisher Scientific) secondary antibodies at 2 mg/ml for 30 min on ice, washed twice, and acquired with a FACS (fluorescence-activated cell sorting) Fortessa (BD Biosciences) flow cytometer.

    Article Title: An Unbiased Screen for Human Cytomegalovirus Identifies Neuropilin-2 as a Central Viral Receptor.
    Article Snippet: .. For cell-surface staining, adherent cells (ARPE-19, HAP-1, HUVEC andMRC-9) were gently detachedwith a cell scraper andwashed twice with PBS+2% FBS, incubated in PBS + 0.5% BSA + 2 mM EDTA with specific anti-Neuropilin-2 and anti-CD46 antibodies (AF2215 and AF2005, R&D systems), anti-Thrombomodulin antibody (clone 141C01, Abcam), anti-TGFbRIII antibody (T1940, Sigma) or, as isotype control, normal sheep IgG affinity pure (R&D systems), goat a-CMV pp71 (vC-20; Santa Cruz Biotechnology) and mouse IgG1 a-CMV pp72 (clone 6E1; Santa Cruz Biotechnology) at 2 mg/ml for 30 min on ice. .. After two washes, cells were incubated with rabbit anti-goat IgG (H+L) Alexa Fluor 594 conjugated or donkey anti-sheep IgG (H+L) Alexa Fluor 488 conjugated or goat F(ab)2 anti-mouse IgG (H+L) Alexa Fluor 594 conjugated (Thermo Fisher Scientific) secondary antibodies at 2 mg/ml for 30 min on ice, washed twice and acquired with a FACS Fortessa (BD Biosciences) flow cytometer.



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    Sino Biological anti human cd46
    (A) A549 cells were infected with GRAd32 or GRAd25 at MOI 50 in the presence (w) or absence (w/o) of an <t>anti-CD46</t> blocking antibody. Images were acquired 3 days post-infection. Competition with CD46 resulted in a delayed onset of cytopathic effect (CPE) exclusively in GRAd25-infected cells. (B) Flow cytometry analysis of surface CAR and CD46 expression levels in lung cancer (A549, NCI-H727, NCI-H1975, NCI-H1299) and normal (MRC5, HUVEC) cell lines. (C) Infection of MRC5 and A549 cells with a replication-defective GRAd32 GFP reporter virus. GFP expression was evaluated at 48 hours post-infection. The presence of GFP signal confirms that GRAd32 is capable of entering MRC5 cells despite its inability to replicate in this cell line.
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    Image Search Results


    (A) A549 cells were infected with GRAd32 or GRAd25 at MOI 50 in the presence (w) or absence (w/o) of an anti-CD46 blocking antibody. Images were acquired 3 days post-infection. Competition with CD46 resulted in a delayed onset of cytopathic effect (CPE) exclusively in GRAd25-infected cells. (B) Flow cytometry analysis of surface CAR and CD46 expression levels in lung cancer (A549, NCI-H727, NCI-H1975, NCI-H1299) and normal (MRC5, HUVEC) cell lines. (C) Infection of MRC5 and A549 cells with a replication-defective GRAd32 GFP reporter virus. GFP expression was evaluated at 48 hours post-infection. The presence of GFP signal confirms that GRAd32 is capable of entering MRC5 cells despite its inability to replicate in this cell line.

    Journal: bioRxiv

    Article Title: A novel Gorilla-derived oncolytic Adenovirus with natural selective replication in cancer cells

    doi: 10.64898/2026.02.26.708271

    Figure Lengend Snippet: (A) A549 cells were infected with GRAd32 or GRAd25 at MOI 50 in the presence (w) or absence (w/o) of an anti-CD46 blocking antibody. Images were acquired 3 days post-infection. Competition with CD46 resulted in a delayed onset of cytopathic effect (CPE) exclusively in GRAd25-infected cells. (B) Flow cytometry analysis of surface CAR and CD46 expression levels in lung cancer (A549, NCI-H727, NCI-H1975, NCI-H1299) and normal (MRC5, HUVEC) cell lines. (C) Infection of MRC5 and A549 cells with a replication-defective GRAd32 GFP reporter virus. GFP expression was evaluated at 48 hours post-infection. The presence of GFP signal confirms that GRAd32 is capable of entering MRC5 cells despite its inability to replicate in this cell line.

    Article Snippet: For the evaluation of CD46 and CAR cell surface levels, 2-3 x 10 5 cells (MRC5, HUVEC, A549, NCI-H1299, NCI-H1975, NCI-H727) were collected and incubated with anti-human CD46 (1:50; mouse monoclonal; #12239-MM05, Sino Biological) or with anti-human CAR (1:50; rabbit monoclonal; #10799-R271, Sino Biological) for 30 min at 4°C.

    Techniques: Infection, Blocking Assay, Flow Cytometry, Expressing, Virus